10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 1 2016 (January - March) Green Coffee Extract (GCE) is a popular health supplement known for its anti-obesity & anti-diabetic effects. Here we tested GCE for its capacity to activate J774.1 mouse macrophages and protect against Doxorubicin induced apoptosis. J774.1 cells treated for 24 h with 100 and 500 µg/ml GCE showed increased production of IL-6, TNF-α and nitric oxide (NO) in a dose dependent manner. GCE upregulated production of these molecules by inducing transcription of the IL-6, TNF-α and iNOS genes. In addition, GCE was able to restore viability of J774.1 cells treated with Doxorubicin. Pre-treatment of cells with GCE for 1 h abrogated Dox induced loss of cell viability. While Dox robustly induced caspase 3/7 activity within 6 h of treatment, pre-treatment with GCE for 1 h was enough to attenuate this induction. The results we report here suggest that GCE activates J774.1 macrophages and is cytoprotective against Dox induced apoptosis. SRIVIDYA MALKAPURAM, SWAMINI TARAN, KRISHNAN VENKATARAMANAND LAKSHMAN RAJAGOPALAN Green Coffee Extract,Doxorubicin, J774.1, macrophages, Caspase 3/7, cytokines, NO, iNOS 153-160