10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 1 2016 (January - March) The development of uniform nanoparticles has been intensively pursued because of their technological and fundamental scientific importance. In this work, we have formulated a simple and effective procedure for biofabrication of silver nanoparticles (AgNPs) using fruit waste extract. The resulting AgNPs were characterized by UV-Visible spectroscopy, Fourier Transform Infra Red (FT-IR) Spectroscopy and Transmission electron microscopy (TEM) techniques. The UV-visible spectra of the silver nanoparticles showed an adsorption peak at 410 nm. The TEM image reveals that the uniformly dispersed AgNPs with a uniform size and shape in the average size of 6 nm. Biosynthesised AgNPs were studied for antimicrobial activity against the human pathogenic bacteria's ( lessThan i greaterThan Proteus, Klebsiella lessThan /i greaterThan ). These nontoxic nanoparticles which can be prepared in a biosynthesis method prove to be potential candidates for medical applications. N. KIRUTHIKA AND T. SOMANATHAN Silver nanoparticles, biofabrication, antibacterial activity, Pathogenic bacteria’s 07-11