10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) The safety of drinking water in an urban city has been challenging since decades. lessThan i greaterThan Salmonellae lessThan /i greaterThan are the enteric pathogens which survive even in treated drinking water. The pathogen harbors lessThan i greaterThan invA lessThan /i greaterThan signature gene responsible for the virulence. Upon infection with lessThan i greaterThan Salmonellae lessThan /i greaterThan , frequent antimicrobial therapy has lead the emergence of drug-resistant strains. The present paper aims to isolate and characterize drug resistant virulent isolates of lessThan i greaterThan Salmonellae lessThan /i greaterThan from drinking water. We have collected drinking water from Gwalior, an important city of Northern India. Out of the five sites, three sites were contaminated with lessThan i greaterThan Salmonellae lessThan /i greaterThan harboring lessThan i greaterThan invA lessThan /i greaterThan gene. All the isolates from sites were resistant against at least 2 antimicrobials. Isolates from site 1, site 2 and site 5 were intermediates (reduced susceptibility) to different antimicrobials. These indicate possibilities of emergence of resistant strains in future. Our observation indicates the presence of drug-resistant isolates in drinking water of Gwalior. This has immense importance for microbial risk assessment. MOHIT AGARWAL, ANURAG JYOTI AND RAJESH SINGH TOMAR Potable water, Salmonellae, virulence determinants, Antimicrobial resistance 997-1004