10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) lessThan i greaterThan Lablab purpureus lessThan /i greaterThan is a protein rich crop plant belonging to the family Leguuminosae. A proteinacious inhibitor of trypsin lessThan i greaterThan ( lessThan /i greaterThan LPTI lessThan i greaterThan ) lessThan /i greaterThan was isolated from lessThan i greaterThan Lablab purpureus lessThan /i greaterThan seeds lessThan i greaterThan . lessThan /i greaterThan Protein was extracted in 0.1 M tris–Cl buffer (pH-8.0) and subjected to precipitation using 20% ammonium sulphate, dialyzed against 0.1M phosphate buffer and further purified by using DEAE cellulose column. Fold purification obtained was 12.0 of LPTI. Three isoforms of LPTI were observed in gelatin embedded native PAGE. SDS-PAGE analysis of lessThan i greaterThan Lablab purpureus lessThan /i greaterThan seed extract eluted from DEAE cellulose column showed three polypeptide bands of~30.1 kDa, ~20.6 kDa and ~17.9 kDa. Solution assay with casein showed 36 mg of inhibitor concentration causes 50% inhibition of trypsin. Thermo stability study showed that the LPTI are quit thermostable, it lost its complete activity when heated at 100°C for 90 minutes. Inhibitors are stable between pH 6.to 8. ANIL S. KHANDAGALE, ADITI A. PATTEWAR AND LAXMIKANT H. KAMBLE* plant seed inhibitor, protease inhibitor, trypsin inhibitor, Lablab purpureus. 763-769