10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) In this work, we reported synthesized silver nanoparticles using the aqueous extract of lessThan i greaterThan Setaria verticillata lessThan /i greaterThan and assessing its antimicrobial activity lessThan i greaterThan . lessThan /i greaterThan The synthesized silver nanoparticles were characterized by UV-Visible spectrophotometry, FT-IR spectroscopy, Scanning electron microscopy and Energy dispersive X-ray analysis. The antimicrobial activity was assessed using agar well diffusion method. The average particle's size of synthesized silver nanoparticles was 12 ± 4 nm. EDAX analysis confirmed the presence of silver nanoparticles Ag (83.02 %). The antimicrobial activity study reveals that the maximum zone of inhibition was observed in lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan and lowest inhibition activity was found in lessThan i greaterThan Escherichia coli. lessThan /i greaterThan The results indicated that lessThan i greaterThan Setaria verticillata lessThan /i greaterThan mediated silver nanoparticles can be synthesized by green chemistry and eco-friendly approach. A. PRABHU, K. SHANKAR , P. MUTHUKRISHNANAND K. PRABHAKARAN Setaria verticillata, silver nanoparticles, EDAX, SEM, FT-IR, antimicrobial activity. 423-429