10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) lessThan i greaterThan Lepidium sativum lessThan /i greaterThan Linn. is a small annual herb placed in the family Cruciferae. It is used in various Ayurvedic drugs in the management of hiccup, diarrhoea, asthma, bronchitis and cough. Seeds are rich source of omega-3 fatty acids, proteins, dietary fiber, iron, other essential nutrients and phytochemicals. In the present paper, methanolic extract of lessThan i greaterThan Lepidium sativum lessThan /i greaterThan seed obtained by Soxhlet extraction was examined for phytochemical screening to detect the phytoconstituents. Pharmacological relevance of the extract for antifungal activity was evaluated at different concentrations of 10, 30, 60 and 90 mg/ml against human pathogenic and opportunistic fungi such as lessThan i greaterThan Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Fusarium lessThan /i greaterThan sp lessThan i greaterThan , Penicillium lessThan /i greaterThan sp lessThan i greaterThan and Penicillium marneffi lessThan /i greaterThan . Imidazole was used as the reference antibiotic. It was observed that methanolic extract showed alkaloids, steroids, terpenoids, flavonoids, phenolic compounds, fixed oils, fats and lessThan i greaterThan Aspergillus flavus lessThan /i greaterThan was the most sensitive fungi, inhibited at 30 mg/ml. ROSHIN ELIZABETH GEORGE, SHIRLY K THOMAS,MANESH KUNJUMON AND THANKAMANI V Lepidium sativum, methanolic extract, phytoconstituents, antifungal. 490-497