10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) Propolis has many advantages and specific potencies, due to its properties as antibacterial, antivirus, and anticancer. Aim of the study is to determine the propolis potency of lessThan i greaterThan Trigona lessThan /i greaterThan spp as antibacterial agent against lessThan i greaterThan E. sakazakii lessThan /i greaterThan . The antibacterial activity test was performed by a modified agar well diffusion against three isolates of bacteria lessThan i greaterThan E. sakazakii lessThan /i greaterThan IB-19b, IB-29a, and ATCC 35217. Through the initiation test, propolis activity was higher than commercial propolis, and lower than ampicillin. The effect of propolis extract to commercial propolis for each isolate IB-19b, IB -29a, and ATCC 35217 were 143.99%, 169.65%, and 155.76% respectively. The effects of propolis extract to ampicillin for each isolate IB-19b, IB-29a, and ATCC 35217 were 39.37%, 38.94%, and 47.37% respectively. The minimum inhibition concentration (MIC) differed for each isolate, the minimum inhibition concentration for IB-19b, IB-29a, and ATCC 35217 were 12.50%, 25.00%, and 50.00% respectively. A.E.Z. HASAN, SURYANI, S. ESTUNINGSIH AND FITRIANNUR propolis, antibacterial, Enterobacter sakazakii, Trigona spp, Indonesia 420-428