10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) The objective of the present work was to develop a simple, precise, cost effective and accurate stability indicating assay method for Zidovudine using UV-Vis spectrophotometer under different stress conditions (hydrolytic, oxidative, photolytic and thermal) recommended by the International Conference for Harmonisation. The stress study was performed by taking distilled water as solvent and absorbance was taken at absorption maxima (λ lessThan sub greaterThan max lessThan /sub greaterThan ) of 267nm. After performing a hydrolytic study of the drug in different conditions of acidic, alkali and neutral medium, the drug was found to be degraded very slowly about 8.7 % in highly acidic condition, 19.5% in alkali media and 5 % in neutral medium. The drug was found to be oxidized by 25.0 % in 3%, H lessThan sub greaterThan 2 lessThan /sub greaterThan O lessThan sub greaterThan 2 lessThan /sub greaterThan  solution, whereas the drug was found to be almost stable in thermal condition but degraded by 15.8% in photolytic condition. A linear response was observed in the range of 5-50μg/ml with a regression coefficient of 0.999. The method was validated as per the ICH (International Conference for Harmonization) guidelines. The % COV value for intermediate precision studies was lessThan 2 indicates the good precision of the method. The % of the recovery of the drug ranged from 100.40 - 102.55 indicates the accuracy of the method for estimation of degradants in different stress conditions. The method was specific to the drug and also selective to degradation products formed under different stressed conditions. DEBADASH PANIGRAHI AND GANESH PRASAD MISHRA Zidovudine, Stability Indicating Assay , Validation , UV- Vis Spectrophotometer. 93-100