10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) lessThan i greaterThan Annona lessThan /i greaterThan produces a novel set of chemicals called annonaceous acetogenins, known to possess antineoplastic activity.The present study was undertaken to establish the antineoplastic potential of lessThan i greaterThan Annona muricata lessThan /i greaterThan by MTT, Cell cycle (G2M) and DNA fragmentation assays. The extracts showed dose dependant growth inhibition of SCC9 (oral cancer), MCF-7 (Breast cancer), A549 (Lung Cancer) and HCT116 (Colorectal Cancer) cells. In contrast to HCT116 cells, the treated SCC9, MCF-7 and A549 cells exhibited significant cytotoxicity at a higher concentration with IC50 value of 87.8, 151.6 and 230.6 respectively. The treated SCC9 and MCF-7cells showed cell cycle arrest up to 27.97% and 19.42% respectively, at G2/M phase of the cell cycle. DNA degradation observed in both MCF-7 and SCC9 cells treated with lessThan i greaterThan A.muricata extract lessThan /i greaterThan at a concentration of 320µg/ml suggest that it might induce apoptosis in both MCF-7 and SCC9 cells. RAJARAJAN.P AND SENTHILKUMAR.R Annona muricata, Antineoplastic activity, MTT, G2M and DNA fragmentation 1101-1109