10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) The study was done as a laboratory based study to detect the carbapenemase production in lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan in a tertiary care hospital. A total of 150 clinically significant consecutive isolates of lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan isolated between October 2012 and March 2013 were included in the study. Isolates from outpatients and from urine samples were excluded. The isolates were from blood (50), respiratory secretions (21) and exudates (79). Antibiotic Susceptibility testing to various drugs was performed by disc diffusion method. Carbapenemase production was screened by Modified Hodge Test (MHT) and lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan carbapenemase(KPC) & Metalobetalactamase(MBL) production by inhibitor potentiated disc diffusion tests with Boronic acid and Ethylene Diamine TetraAcetic acid(EDTA) respectively. The isolates were subjected to Polymerase Chain Reaction (PCR) for the detection of lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan NDM lessThan /sub greaterThan , lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan VIM lessThan /sub greaterThan , lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan IMP lessThan /sub greaterThan , lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan OXA-48 lessThan /sub greaterThan lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan OXA-181 lessThan /sub greaterThan & lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan KPC lessThan /sub greaterThan .Out of 150 isolates, 58(39%) exhibited carbapenem resistance. MHT was positive in 49(32.6%) isolates. Among the 49 MHT positive isolates 30 carried MBL genes. Among the 42 MBL screening was positive isolates 37 carried MBL genes. Overall, lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan NDM-1 lessThan /sub greaterThan , lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan VIM lessThan /sub greaterThan & lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan IMP lessThan /sub greaterThan genes were detected in 34, 1 & 2 isolates respectively. MIC lessThan sub greaterThan 50 lessThan /sub greaterThan to imipenem was 0.125µg/ml lessThan i greaterThan . lessThan /i greaterThan lessThan i greaterThan Bla lessThan /i greaterThan lessThan sub greaterThan KPC lessThan /sub greaterThan , lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan OXA-48 , lessThan /sub greaterThan lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan OXA-181 lessThan /sub greaterThan were not detected. lessThan i greaterThan Bla lessThan /i greaterThan lessThan sub greaterThan NDM-1 lessThan /sub greaterThan is the main mediator of carbapenem resistance in lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan followed by lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan VIM lessThan /sub greaterThan & lessThan i greaterThan bla lessThan /i greaterThan lessThan sub greaterThan IMP lessThan /sub greaterThan . Carbapenemase production (63.7%) is the most important reason for carbapenem resistance in carbapenem resistant lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan . PCR remains the gold standard for the detection of carbapenemase production. ABIRAMASUNDARI VK, UMA SEKAR, SHANTHI.M AND ARUNAGIRI .K Klebsiella pneumoniae, carbapenem resistance, Modified Hodge test, Inhibitor potentiated disc diffusion 847-856