10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) The current study is conducted to evaluate the enzymatic and non-enzymatic antioxidant activity of lessThan i greaterThan Jatropha curcas lessThan /i greaterThan leaves methanolic extract. Additionally, evaluation the changes in gene expression and DNA damage in female rat liver after lessThan i greaterThan intraperitoneally lessThan /i greaterThan injection of carbon tetrachloride (CCl lessThan sub greaterThan 4 lessThan /sub greaterThan ) as well as demonstration the possible therapeutic effects of lessThan i greaterThan J. curcas lessThan /i greaterThan extract in comparison with the reference hepatoprotective drug; silymarin were studied. The present results declare that, injection of rats with CCl lessThan sub greaterThan 4 lessThan /sub greaterThan twice a week for six consecutive weeks induced hepatic toxicity. However, treatment of intoxicated rats with 250 mg/kg body weight daily for one month with methanolic extract of lessThan i greaterThan J. curcas lessThan /i greaterThan showed enhancement in the glutathione (GSH), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) levels with percentages of improvement reached to 28.34, 12.78, 47.88 and 51.65%, respectively. In addition, the results indicated that treatment of female rats with CCl lessThan sub greaterThan 4 lessThan /sub greaterThan significantly increased the DNA damage in liver cells and significantly induced alterations of the genes expression encoding antioxidant enzymes; superoxide dismutase (SOD), CAT and GPx as well as HSP70 proteins in liver tissues as compared to control rats. While, treatment with lessThan i greaterThan J. curcas lessThan /i greaterThan extract as compared to silymarin decreased significantly the rate of the DNA damage and suppressed the alterations in the gene expression of rats induced by CCl lessThan sub greaterThan 4 lessThan /sub greaterThan . Thus, the present results strongly suggeste that, lessThan i greaterThan J. curcas lessThan /i greaterThan extract has markedly ameliorative role associated with CCl­­ lessThan sub greaterThan 4 lessThan /sub greaterThan -induced lessThan sub greaterThan geno and lessThan /sub greaterThan hepatotoxicity in rats. FAROUK K. EL-BAZ , WAGDY K.B. KHALIL,HANAN F. ALY, HODA F. BOOLES AND SAFAA A. SAAD Jatropha curcas, Antioxidant enzymes, Gene expression, DNA damage 767-781