10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) Distant geographical niches have shown a pronounced effect on variability in organoleptic characteristics as well as biochemical constituents between accessions of a given plant species, keeping this in view the present study has been designed using five accessions of memory enhancing herb lessThan i greaterThan Bacopa monnieri lessThan /i greaterThan . Maximum friable callus was regenerated in accession BM003 (GI: 54.61 ± 0.11) on B lessThan sub greaterThan 5 lessThan /sub greaterThan media supplemented with 2,4-D (1.0 mg l lessThan sup greaterThan -1 lessThan /sup greaterThan ) while, MS media with NAA (1.0 mg l lessThan sup greaterThan -1 lessThan /sup greaterThan ) + BAP (0.5 mg l lessThan sup greaterThan -1 lessThan /sup greaterThan ) resulted in maximum indirect organogenesis in BM003 (GI: 99.58 ± 0.06). Biochemical profiling proved the qualitative differences in the carbohydrate, alkaloid, saponin and terpenoid contents in leaf, shoot and root samples of all accessions. LC-ESI-MS analysis revealed that the shoot extract of accession BM002 (7236.38 µg gm lessThan sup greaterThan -1 lessThan /sup greaterThan ) contains highest Bacoside content as compared to that of its root extract (1015.32 µg gm lessThan sup greaterThan -1 lessThan /sup greaterThan ) and the shoots had higher Bacoside contents as compared to the roots. A. KOUL, M. KUSHWAHA, A.P. GUPTA AND S. MALLUBHOTLA Brahmi, micropropagation, biochemical profiling, Liquid chromatography-Electro spray ionization-Mass spectrometry. 746-756