10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) The frequent, increasing drug resistance among urinary tract infections (UTIs) causing bacteria has made therapy difficult and also lead to greater use of expensive broad-spectrum drugs. The aim of the study was to assess the antimicrobial activity of lessThan i greaterThan Tinospora cordifolia lessThan /i greaterThan stem extracts, a broad-spectrum therapeutic plant investigated against bacteria causing UTIs, most common diseases infecting from the neonate to the geriatric age groups. The two isolated uropathogens:1.Gram-negative, lessThan i greaterThan Escherichia coli; lessThan /i greaterThan and 2.Gram-positive, lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan were tested against two standard antibiotics, used as a positive reference to determine the sensitivity of the test strains. The extracts of lessThan i greaterThan T. cordifolia lessThan /i greaterThan were prepared from alcoholic extracts, and water reflux for aqueous extract. The antibacterial activities of extracts were evaluated using the disc diffusion method. The study showed that all three solvent extracts of lessThan i greaterThan T. lessThan /i greaterThan lessThan i greaterThan cordifolia lessThan /i greaterThan reveal different antibacterial activity against both uropathogenic isolates. The antibacterial effect of lessThan i greaterThan T. cordifolia lessThan /i greaterThan , which were observed for zone of inhibition (ZOI) could be arranged in decreasing order as follows: ethanolic (maximum) greaterThan methanolic (moderate) greaterThan aqueous (poor). The evaluated results reveals that plant extracts have significant potential of antibacterial properties and seems promising for the development of the safe herb derived medicinal preparation for treating UTIs. PRIYANKA SINGH, DEEPMALA KATIYAR, BHARTI SINGH AND APARNA SRIVASTAVA Antimicrobial activity; Tinospora cordifolia; Urinary tract infections (UTIs); Disc diffusion assay; Uropathogen; Zone of inhibition (ZOI). 571-577