10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) The genus lessThan i greaterThan Hypericum lessThan /i greaterThan consists of both advanced and primitive tribes and taxa containing a complex mixture of secondary metabolites many of which are still being investigated. In the present study, we analyzed the methanol extracts of three lessThan i greaterThan Hypericum lessThan /i greaterThan species ( lessThan i greaterThan H.hookerianum, H.mysorense, and H.wightianum lessThan /i greaterThan ) of the Palni hills in southern India for the presence of bioactive hypericin and hyperforin and found characteristic discrimination of the three species based on LC-MS profiles. We report for the first time that lessThan i greaterThan H.mysorense lessThan /i greaterThan lacks both these metabolites, lessThan i greaterThan H.wightianum lessThan /i greaterThan contains only lessThan i greaterThan hypericin lessThan /i greaterThan while lessThan i greaterThan H.hookerianum lessThan /i greaterThan contains both the metabolites. NARAYANAN RAVISANKAR AND JERRINE JOSEPH Hypericum, LC-MS, H.hookerianum, H.mysorense, and H.wightianum 373-383