International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 6 Issue 3
2015 (July - September)
ANTIOXIDANT POTENTIAL ACTIVITY AND CYTOTOXICITY EFFECTS OF DIFFERENT PARTS OF PEANUTS (ARACHIS HYPOGAEA L.)
Peanut skin and peel, a low economic value by-product of the peanut industry, is rich in potentially health promoting compounds. This work aimed to evaluate the efficiency of different organic solvents such as, methanol, ethanol, acetone, and aqueous extract for extraction of (total phenolics TP, total flavonoids TF and total tannins TT) from the peanut skin and peel. For each solvent, the content of total phenolics, flavonoids, tannins was determined. Antioxidant activity of different extracts was screened using the 2, 2-diphenyl-1-picryl hydrazyl (DPPH lessThan sup greaterThan • lessThan /sup greaterThan ) radical scavenging, metal chelating activities, reducing power, and ABTS assays. The results showed that 80% acetone is the best solvent for the extraction of total phenolics (436.88 ± 0.71mg/g DW), flavonoids (340.00 ± 1.11mg/g DW) and tannins (184.63 ± 1.95 mg/g DW) and lower in peanut peel aqueous extract (135.5 ± 1.05 mg/g DW), (163 .15 ± 0.67mg/g DW) and (90.55 ± 3.63 mg/g DW) lessThan i greaterThan . lessThan /i greaterThan The results revealed that all extracts of the peanut skin and peel exhibited variable antioxidant activity. The highest DPPH lessThan sup greaterThan • lessThan /sup greaterThan scavenging activity (IC lessThan sub greaterThan 50 lessThan /sub greaterThan =52.18 ± 0.47µg/ml) was achieved with acetone extracts of peanut skin compared to acetone extract of peanut peel (IC lessThan sub greaterThan 50 lessThan /sub greaterThan =70.51 ± 0.45µg/ml). Antioxidant as measured by Fe lessThan sup greaterThan 2+ lessThan /sup greaterThan -chelating revealed the highest activities with methanolic extract of peanut skin (86.10%) compared to peel extract (74.94%). ABTS lessThan sup greaterThan •+ lessThan /sup greaterThan showed the highest value with peanut skin and peel methanol extract. While the acetone skin extract showed the highest activity by reducing power assay. The present study suggests that a skin peanut acetone extract contained higher antioxidant components and potential as compared to the peel. Peanut skin acetone extract exhibited potential as an anticarcinogenic activity, but needs further investigation. It is concluded that acetone and methanol are polar among the solvent used and play a vital role in the extraction of the plant constituents. Therefore these results suggested that peanut skin might be beneficial as a potent antioxidant and anticancer agents and effectively employed as an ingredient in food applications.
ALAA A. GAAFAR, KHALED M. MAHMOUD AND ZEINAB A. SALAMA
Antioxidants, DPPH•, Iron chelating, reducing power, ABTS•+, anticancer, peanut skin and peanut peel.
19-32