10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) Proteases are proteolytic enzymes secreted by most pathogenic microorganisms during their mode of entry into the host system. Many pathogenic bacteria such as lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan produce proteases during infection and due to their emergence as antibiotic resistance, pose problems and threat for efficient control of bacterial diseases. To control such pathogenicity, mediated by the secretion of proteases, protease inhibitor of microbial origin from medicinal plant, lessThan i greaterThan Justicia lessThan /i greaterThan lessThan i greaterThan wynaadensis lessThan /i greaterThan plant-rhizosphere microorganisms were screened, to check their possible role in inhibiting the proteolytic activity and antibacterial effect against the pathogenic organisms such as lessThan i greaterThan Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas sp, and Escherichia coli. lessThan /i greaterThan Broth dilution assays were performed to check antibacterial activity.  RAJESHWARI.K AND MANJULA.I.K Cysteine proteases, Inhibition, Justicia wynaadensis, Rhizosphere-bacterial isolates, Antibacterial, Broth dilution. 131-138