10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 3 2015 (July - September) In the present investigation, a field experiments aimed at evaluating phytoconstituents, chemical composition, antioxidants and antimicrobial activity of lessThan i greaterThan V. elaeagnifolia lessThan /i greaterThan leaf. The gas chromatography coupled to mass spectroscopy GC-MS, eight compound were determined, the major compounds identified in the crude plant extract were Phorbol12,13- dihexanolate(19.84%), Cyclopropal (3,4) benz (1,2,e) azulene- (16.55%), Mibemycin, b,13- choro-5-demethoxy-28-epoxy-5(hydroxyimino)-25-(1-methylethy)(15.12%).The timicrobial susceptibility results obtained in this work revealed that the extraction yield of the different extraction solvents used was correlated with the antimicrobial activity observed. Of the isolates tested, only 3(MEE and EAE) revealed susceptibly to the extracts, with DMSOE revealing the highest susceptibility. DPPH radical scavenging activity of lessThan i greaterThan Vernonia lessThan /i greaterThan leaf extract with ascorbic acid as reference where the IC lessThan sub greaterThan 50 lessThan /sub greaterThan value for the lessThan i greaterThan Vernonia lessThan /i greaterThan extract (IC lessThan sub greaterThan 50 lessThan /sub greaterThan = 3259 µg/ml) and IC lessThan sub greaterThan 50 lessThan /sub greaterThan value was said to be moderate as compared to standard (IC lessThan sub greaterThan 50 lessThan /sub greaterThan = 2782 µg/ml). U.S. KHANDEKAR , S.K. TIPPAT, R.A. GHONGADE AND K. DUDHE V. elaeagnifolia, Antimicrobial, DPPH, GC-MS 07-15