10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 2 2015 (April - June) The search for new substances with antimicrobial properties has become necessary due to the microbial capacity of resistance. Medicinal plants have become an important substitute since many plants exhibit antimicrobial activity. In this study, the ethanolic flower extracts of lessThan i greaterThan Punica granatum lessThan /i greaterThan were assessed for their phytoconstituents and the antimicrobial property. A total of 21 microorganisms (19 bacteria and 2 fungi) were used for antimicrobial activity by disc diffusion method, broth dilution method and a standard procedure was employed to identify the Phytoconstituents. The Ethanolic flower extract showed significant inhibitory activity against lessThan i greaterThan Salmonella paratyphi lessThan /i greaterThan A (25.6 mm), lessThan i greaterThan Salmonella typhimurium lessThan /i greaterThan , lessThan i greaterThan Salmonella enterica lessThan /i greaterThan (22.8 mm), lessThan i greaterThan Yersinia enterocolitica lessThan /i greaterThan (22 mm), lessThan i greaterThan Staphylococcus epidermidis lessThan /i greaterThan (21 mm), lessThan i greaterThan Salmonella brunei lessThan /i greaterThan (20.6mm), lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan (20 mm) and lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan , lessThan i greaterThan Burkholderia cepacia lessThan /i greaterThan (19.6 mm) mean values were documented. Based on the broth dilution method, the ethanolic flower extract showed the MIC value 256µg/ml against lessThan i greaterThan Salmonella paratyphi lessThan /i greaterThan A with a significant inhibitory activity. Ethanolic flower extract of lessThan i greaterThan P. granatum lessThan /i greaterThan showed the highest antibacterial activity when compared to the antifungal activity could be due to the presence of some active phytoconstituents. KANNAIYAN MOORTHY, THAMBIDURAI PUNITHA AND RAJA VINODHINI P. granatum, Phytoconstituents, MIC, Antibacterial and Antifungal activity 1155-1164