10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 2 2015 (April - June) The aim of the present study is to determine the antioxidant, carotene and alkaloids content of the selected plant leaves namely lessThan i greaterThan spinach oleracea lessThan /i greaterThan and lessThan i greaterThan Annona squamosa. lessThan /i greaterThan The antioxidant screening was done by DPPH free radical scavenging assay using shade dried leaf extracts. The results showed that IC lessThan sub greaterThan 50 lessThan /sub greaterThan for lessThan i greaterThan Annona squamosa lessThan /i greaterThan and lessThan i greaterThan Spinach oleracea lessThan /i greaterThan was 68.24 (µg/ml), 42.57 µg/ml respectively. Annona squamosa shows highest free radical scavenging activity at the concentration 20µg/ml. lessThan i greaterThan Annona squamosa lessThan /i greaterThan was found to have a maximum amount of carotene (173.24 mg/100gm) followed by lessThan i greaterThan Spinach oleracea lessThan /i greaterThan (58.88 mg/100gm). Regarding alkaloid estimation, lessThan i greaterThan Annona squamosa lessThan /i greaterThan has the highest alkaloid content of 0.48%, while lessThan i greaterThan Spinach oleracea lessThan /i greaterThan had the lowest content (0.03%). Plant antioxidants are believed to play a role in protection against various diseases and delaying ageing processes. Hence the present investigation suggests that medicinal plants which possess good antioxidant potential are the best supplements for the diseases associated with oxidative stress. V.KAVITHA AND V.SARADHA RAMADAS Spinach oleracea, Annona squamosa, Antioxidant, Carotene, Alkaloid 1071-1076