10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 2 2015 (April - June) The objective of the current study is to develop a validated RP- HPLC method for the simultaneous estimation of Zidovudine lessThan sup greaterThan 1 lessThan /sup greaterThan and Lamivudine lessThan sup greaterThan 2 lessThan /sup greaterThan in tablet dosage form. The method follows sample preparation step followed by separation on Phenomenex C18 column (250 x 4.6mm id, 5 μm particle size utilizing Shimadzu HPLC (LC-10AT VP) equipped with UV-Visible and PDA detector. The mobile phase was prepared by mixing methanol, acetonitrile and water in the ratio of 60:20:20v/v/v. The eluted peaks were detected by photo diode array (PDA) detector at a wavelength of 265nm. The method was validated with respect to linearity, accuracy, precision, and robustness. The utility of the procedure was verified by its application to formulations and it was found that the developed method is fast, accurate, precise, selective, reproducible and cost effective and can be used for the routine laboratory analysis of tablet dosage form in institutions and industries. RICHA. A. DAYARAMANI AND PARESH U PATEL Zidovudine and Lamivudine, NRTI, RP-HPLC, simultaneous estimation, method development and validation, pharmaceutical dosage form 330-342