10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 2 2015 (April - June) Arbuscular mycorrhizal fungi (AMF) are associated with almost all plants in nature and are ubiquitous. The arbuscular mycorrhizal fungi status of 10 wild herb and shrub was investigated during winter and summer season in Pine forest of himalaya(HP). The herbs and shrubs investigated for studied are: lessThan i greaterThan Reinwardtia indica, Berberis lycium, Viola serpens,Taraxacum officinale, Caryopteris wallichiana, Indigofera pulchella, Rumex hastatus, Colebrookea oppositifolia, Leucas lantata lessThan /i greaterThan and lessThan i greaterThan Verbascum thapsus lessThan /i greaterThan . The result indicated that in all the ten selected wild herb and shrub Arbuscular mycorrhizal fungi (AMF) were observed in both winter and summer season. In the present study, the percentage of AMF root colonization was less in summer and higher in winter season. Similarly the highest number of AMF spore was found in winter and least in summer. In winter season, the maximum percent root colonization of AM fungi was observed in lessThan i greaterThan Verbascum thapsus lessThan /i greaterThan (83.33±4.17) while minimum in lessThan i greaterThan Indigofera pulchella lessThan /i greaterThan (45.83±4.17). The maximum AMF spore count from 50g rhizosphere soilwas observed in lessThan i greaterThan Indigofera pulchella lessThan /i greaterThan (744.67±2.67) while minimum in lessThan i greaterThan Leucas lantata lessThan /i greaterThan (254.67±2.40). In summer season, the percent root colonization of AM fungi was observed equal in lessThan i greaterThan Reinwardtia indica lessThan /i greaterThan (58.33±4.17), lessThan i greaterThan Berberis lycium lessThan /i greaterThan (58.33±4.17), lessThan i greaterThan Viola serpens lessThan /i greaterThan (58.33±4.17), lessThan i greaterThan Leucas lantata lessThan /i greaterThan (58.33±4.17). It was minimum in lessThan i greaterThan Caryopteris wallichiana lessThan /i greaterThan (33.33±4.17).Maximum AMF spore counts from 50g rhizosphere soil were observed in lessThan i greaterThan Taraxacum officinale lessThan /i greaterThan (543±2.64) while minimum in lessThan i greaterThan Leucas lantata lessThan /i greaterThan (153.67±1.85). A total of 25 AMF species were isolated which belonged to five genera, i.e., lessThan i greaterThan Glomus, Acaulospora, Gigaspora, Endogone lessThan /i greaterThan and lessThan i greaterThan Dentiscutata lessThan /i greaterThan s in both winter and summer seasons. lessThan i greaterThan Glomus lessThan /i greaterThan was reported to be dominant generafollowed by lessThan i greaterThan Acaulospora lessThan /i greaterThan in winter and summer seasons.Results showed area as a rich source of varied arbuscular mycorrhizal fungi. REENU KUMARI, SUNIL PURI AND MAMTA SHARMA Seasonal variation, mycorhiza, AMF, root colonization. 140-160