IDENTIFICATION AND QUANTIFICATION OF PHYTOCOMPOUNDS IN ACALYPHA INDICA LEAVES

10.22376/ijpbs.2019.10.1.p1-12

Volume 6 Issue 2 2015 (April - June) IDENTIFICATION AND QUANTIFICATION OF PHYTOCOMPOUNDS IN ACALYPHA INDICA LEAVES lessThan i greaterThan Acalypha indica lessThan /i greaterThan , a known medicinal plant in the treatment of various degenerative diseases and a potential source of valuable bioactives. The present study investigates the total phenolic and flavonoid content and also screen for phytocompounds in lessThan i greaterThan A. indica lessThan /i greaterThan leaf extract obtained by two extraction methods, comparatively. Results indicated that the maceration extraction method (MEM) had highest total phenolic content and the sequential extraction method (SEM) showed highest total flavonoid content in methanolic extracts. RP-HPLC (Reversed Phase-HPLC) analysis revealed the presence of gallic acid, caffeic acid, 3,3´-Methylene-bis(4-hydroxycoumarin), syringic acid, ferulic acid, rutin and naringenin in methanolic extract lessThan i greaterThan . lessThan /i greaterThan Major phenolic acid and flavonoid identified were gallic acid (119.7 mg kg lessThan sup greaterThan -1 lessThan /sup greaterThan dry weight of extract) and naringenin (125.29 mg kg lessThan sup greaterThan -1 lessThan /sup greaterThan ) in lessThan i greaterThan A. indica lessThan /i greaterThan extract, respectively. Thus, the results suggest that these identified phenolic compounds makes lessThan i greaterThan A. indica lessThan /i greaterThan a good source of bioactive phenolic compounds and provide desirable health benefits beyond basic nutrition. PONNIAH SENTHIL MURUGAN AND GOVINDAN SADASIVAM SELVAM Acalypha indica; Total phenolic content; Total flavonoid content; phenolic acids; flavonoids; RP-HPLC 11-21