International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 6 Issue 1
2015 (January - March)
HALOSTABLE ALKALINE AMYLASE AND PROTEASE FROM AN EXTREMELY HALOALKALIPHILIC ARCHAEON, NATRINEMA SP. SSBJUP-1 ISOLATED FROM LONAR LAKE.
An extremely haloalkaliphilic archaeon lessThan i greaterThan Natrinema lessThan /i greaterThan sp. SSBJUP-1 showing potent amylolytic and proteolytic activities was isolated from the saline alkaline Lonar Lake, India. Amylase and protease were active over the neutral to alkaline pH range from 6 to 9. Amylase was more stable over a narrower temperature range of 40-60 lessThan sup greaterThan o lessThan /sup greaterThan C than the protease but had the higher optimum (55 lessThan sup greaterThan o lessThan /sup greaterThan C) for activity. By contrast, protease was active over a broader range 10-75 lessThan sup greaterThan o lessThan /sup greaterThan C with an optimum of 40 lessThan sup greaterThan o lessThan /sup greaterThan C. The amylase was stable over a broader salinity range, but with an optimum salt concentration of 10-12%, whereas the protease was with a higher optimum concentration of 18% salt in the reaction mixture. Calcium and Magnesium behaved as activators for both enzymes while Copper was an activator for protease while being an inactivator for amylase. Amylase was highly tolerant to five commercial detergents tested while the protease was tolerant only to one.
J. U. PATIL AND S. S. BAJEKAL
Haloalkaliphiles, Archaea, Natrinema sp, Amylase, Protease.
777-786