10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) The antibacterial activity of lessThan i greaterThan Wrightia lessThan /i greaterThan lessThan i greaterThan tinctoria lessThan /i greaterThan (Roxb.) R. Br. callus extract was studied against selected pathogenic bacterial strains, lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan , lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan and lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan by Agar disc diffusion method. Among the three solvents and water were used , methanol extract of callus showed higher inhibition zone against lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan (27 mm), lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan (22 mm), lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan (20 mm) and lessThan i greaterThan Escherichia coli lessThan /i greaterThan (07 mm). The level of inhibitory activity varies widely with different bacterial species for different solvents and water extract. T. GEETHA, N. KOMALAVALLI AND S. SIVA SUBRAMANIAN Antibacterial activity, Callus extract, Pala indigo plant. 1137-1141