International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
STATISTICAL OPTIMISATION OF THE CRUDE MEDIA AND COMPONENTS FOR ENHANCED PRODUCTION OF BACTERIOCIN FROM BACILLUS SPECIES BY PLACKETT- BURMAN DESIGN.
A lessThan i greaterThan Bacillus lessThan /i greaterThan strain producing a bacteriocin-like substance was isolated from mangrove soil & identified by biochemical profiling and 16S rRNA sequencing. Phylogenetic analysis indicated that the strain has high sequence similarity with lessThan i greaterThan Bacillus licheniformis. lessThan /i greaterThan The culture supernatant of lessThan i greaterThan B. licheniformis lessThan /i greaterThan exhibited bacteriocin-like activity against Gram-positive and Gram negative bacteria and fungi. Enhancement of bacteriocin activity was tested using cheap crude sources like molasses, corn steep liquor and cheese whey. Corn steep liquor and cheese whey is rich nitrogen sources rather than carbon sources. Selection of the crude media sources was done by Students t-test where corn-steep liquor for lessThan i greaterThan Bacillus licheniformis lessThan /i greaterThan was found to be significant. Optimization using Plackett-Burman design showed Sodium chloride, potassium nitrate, sugar,as the most important variables for bacterocin production in the first step of media. The stability study shows bacteriocin produced by lessThan i greaterThan Bacillus licheniformis lessThan /i greaterThan was stable over a wide pH (3-11) and temperature [4-37 lessThan sup greaterThan 0 lessThan /sup greaterThan C]. A low-molecular-weight bacteriocin-like protein from lessThan i greaterThan B. licheniformis lessThan /i greaterThan exhibited a wide spectrum of antimicrobial activity. A 2-fold increase in the production of bacteriocin was achieved using the culture medium optimized through Plackett-Burman design.
SUPRIYA KAMATHE AND PROF. SAVANTA V. RAUT
Bacillus spp, Bacteriocins, Characterisation, Medium Optimisation, Plackett-Burman design, Stability.
815-824