10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) The present study was to compare the Phytochemical and Antimicrobial analysis of lessThan i greaterThan W. somnifera lessThan /i greaterThan and lessThan i greaterThan W. obtusifolia lessThan /i greaterThan . In the Ethanolic leaf and root extract of lessThan i greaterThan Withania lessThan /i greaterThan species were screened for phytochemical compounds by qualitative and FT-IR, UV method. lessThan i greaterThan Withania lessThan /i greaterThan species have been used for remedies of human pathogen because they contain therapeutic compounds. This research work was mainly focused to indicate on the identification of electron transition and the functional group of phytochemical constitute like wise FT-IR and UV spectroscopy. A. RAMACHANDRAN AND M. SENTHIL KUMAR W. somnifera, W. obtusifolia, Antimicrobial activity, FT-IR, UV-Visible spectroscopy analysis 111-117