International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
FORMULATION, CHARACTERIZATION AND IN-VITRO EVALUATION OF RESEALED ERYTHROCYTES AS A CARRIERFOR ANTINEOPLASTIC AGENTS
Erythrocytes have been extensively studied for their potential carrier capabilities. Drug-loaded carrier erythrocytes are prepared by collecting blood samples,separating erythrocytes from plasma, entrapping drug in the erythrocytes, and resealing the loaded cells. The process is based on the response of these cells under osmotic conditions. Paclitaxel is an anticancer agent use for treating breast cancer and cervical cancer. The study involved the usage of cross linking agents like DMF, DMSO and Glutaraldehyde for the formulation of Paclitaxel resealed erythrocytes. The procedure considered for the formulation is Preswell dilution technique as this method led to high entrapment efficiency. The formulated resealed erythrocytes were evaluated for parameters like scanning electron microscopy, zeta Potential, particle size analysis, drug assay, osmotic fragility studies, turbulence fragility studies, osmotic shock studies, percentage haemoglobin release and in-vitro drug release studies and all the values were found to be within the prescribed limits .Upon observing all the parameters ,formulation prepared by using Glutarldehyde as a cross linking agent was found to be the ideal and best formulation, followed by formulations containing DMF and DMSO as cross linking agent.
RANUKA SARMA MALLELA
Resealed erythrocytes, Crosslinking agents, Osmotic conditions, Gluataraldehyde, Paclitaxel, Preswell Dilution technique
86-109