International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 3
2014 (July- September)
EFFECT OF DRIED WHOLE PLANT POWDER OF LEPTADENIA RETICULATA (RETZ.) WIGHT & ARN. AND PLUCHEA LANCEOLATA C.B. CLARKE ON PHAGOCYTOSIS AND RESPIRATORY BURST BY HUMAN POLYMORPHONUCLEAR NEUTROPHILS (IN VITRO STUDY)
In the present study, the effects of hydroalcoholic extract of whole plant powder of lessThan i greaterThan Leptadenia reticulata lessThan /i greaterThan (Retz.) Wight & Arn. and lessThan i greaterThan Pluchea lanceolata lessThan /i greaterThan (DC.) C.B. Clarke was explored on the respiratory burst in Polymorphonuclear Neutrophils (PMNs), as compared to a known stimulant PMA (Phorbol 12-myristate 13-acetate). Prior to the lessThan i greaterThan in vitro lessThan /i greaterThan tests, the viability of the PMNs was assessed using the Trypan blue dye exclusion test. The formation of various Reactive Oxygen Species (ROS) was measured by performing lessThan i greaterThan in vitro lessThan /i greaterThan assays viz. Phagocytosis of lessThan i greaterThan Candida albicans lessThan /i greaterThan , Nitro blue Tetrazolium (NBT) assay and Nitric oxide (NO) assay. The studied concentration of of hydroalcoholic extract of whole plant powder of lessThan i greaterThan Leptadenia reticulata lessThan /i greaterThan (Retz.) Wight & Arn. and lessThan i greaterThan Pluchea lanceolata lessThan /i greaterThan (DC.) C.B. Clarke were 50, 100, 200, 400mg/ml. An increase in the respiratory burst at all the studied concentrations was observed in all the assays indicating its immunostimulating effect
DR. VIDYA DIGHE , SAIKAT S. MALLICK AND HINA Q. SHAIKH
Immunomodulatory Leptadenia reticulata (Retz.) Wight & Arn. Pluchea lanceolata (DC.) C.B. Clarke. Phagocytosis respiratory burst human Polymorphonuclear Neutrophils
790-798