10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) Pulp and paper mill effluent is posing a serious threat to the water and soil components of the environment due to its brown colour and high level of toxicity. Fungal treatment is one of the efficient methods for treating pulp and paper mill effluent. In the present study, for the treatment of from pulp and paper industry, nine efficient fungal strains including lessThan i greaterThan Phanerochaete chrysosporium lessThan /i greaterThan , dedicated for the selective degradation of lignin, have been isolated. Enzyme activities (Fpase, xylanases, lignin peroxidases and carboxy methyl cellulase) required for the degradation of lignocellulosic waste, protein and reducing sugar content were determined. Among all the strains, F lessThan sub greaterThan 3 lessThan /sub greaterThan ( lessThan i greaterThan Paecilomyces lessThan /i greaterThan sp.) strains showed highest activity of lignin peroxidase (3.3 IUml lessThan sup greaterThan -1 lessThan /sup greaterThan ), Fpase (1.2 IUml lessThan sup greaterThan -1 lessThan /sup greaterThan ), xylanase (0.85 IUml lessThan sup greaterThan -1 lessThan /sup greaterThan ), CMcase (0.49 IUml lessThan sup greaterThan -1 lessThan /sup greaterThan ), protein (2.8 mgml lessThan sup greaterThan -1 lessThan /sup greaterThan ), and sugar content (2.1 mgml lessThan sup greaterThan -1 lessThan /sup greaterThan ) at 120 h incubation period. Enzyme extracts of these fungal isolates were applied for the treatment of pulp and paper mill effluent. Data indicated that among all the strains, lessThan i greaterThan Paecilomyces lessThan /i greaterThan sp. (F lessThan sub greaterThan 3 lessThan /sub greaterThan ) showed highest removal of colour (80 per cent) and other pollution parameters (73 per cent lignin, 78 per cent COD and 76 per cent AOX) in pulp and paper mill effluent, on day 3 at 3.3 international unit per ml concentration of crude enzymes. PRATIBHA SINGH AND ASHIMA SRIVASTAVA Lignin peroxidase, pulp and paper mill, effluent treatment 773-783