10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) lessThan i greaterThan Cichorium intybus lessThan /i greaterThan is a medicinal plant commonly used in traditional medicine to treat various infectious diseases. In the present investigation, coumarin was detected in various extracts. An RP- HPLC procedure suitable for the determination of the considered compounds was also developed. Studies were carried out on analysis of secondary metabolites by RP-HPLC from different parts of lessThan i greaterThan C.intybus lessThan /i greaterThan . HPLC detected the coumarin from lessThan i greaterThan C lessThan /i greaterThan . i lessThan i greaterThan ntybus lessThan /i greaterThan in various part of the plant and leaf derived callus. The plant parts used for the analysis included leaf, root and leaf derived callus. Among all the plant parts used, leaf derived callus recorded the highest concentration of coumarin at 219.73µg/ml. The study indicated that almost all parts of the plant contain coumarin component. DAKSHAYINI AND UJWAL P Cichorium intybus, HPLC, secondary metabolite, extraction technique, coumarin. 640-646