10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) Linezolid is one of the most potent antibacterial agents against gram positive organisms especially used in the treatment of skin and soft tissue infections and hospital acquired pneumonias. Recently resistant isolates to this drug are increasingly observed in many parts of the world but resistance is still rare in India. We have encountered two such resistant isolates from a tissue specimen culture. The isolates were identified as lessThan i greaterThan S.aureus lessThan /i greaterThan and lessThan i greaterThan S.epidermidis lessThan /i greaterThan by phenotypic tests. Both the isolates were resistant to linezolid by disc diffusion method. When the minimum inhibitory concentration (MIC) was determined by E- strip method, MIC for lessThan i greaterThan S.aureus lessThan /i greaterThan was 32µg/ml and that of lessThan i greaterThan S.epidermidis lessThan /i greaterThan was greaterThan 256 µg/ml. lessThan i greaterThan cfr lessThan /i greaterThan gene was detected in both the isolates by PCR. Both the isolates were sensitive to Tigecycline, Vancomycin, Cotrimoxazole and Levofloxacin with variable resistance to other antibiotics. This is a first case report of lessThan i greaterThan cfr lessThan /i greaterThan gene mediated linezolid resistance in our country. S.BASIREDDY, M.SINGH, S. ALI AND V. KABRA Linezolid resistance, Staphylococci, cfr gene, MIC, PhLOPSA phenotype. 129-135