10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) Prodigiosin are a group of natural pigments produced by lessThan i greaterThan Serratia marcescens. lessThan /i greaterThan The pigment is produced by various bacteria like lessThan i greaterThan Pseudomonas magneslorubra, Vibrio psychroerythrous, Streptomyces spectabilis, etc., lessThan /i greaterThan This pigment is known for its anti bacterial, antifungal, antiprotozoan and anticancer activity. The present investigation focuses on the optimization of cultural parameters like incubation period, pH, oils, cultural media, and carbon and nitrogen sources, to enhance the production of Prodigiosin from lessThan i greaterThan S.marcescens lessThan /i greaterThan , followed by its chromatographic analysis. DR. D. GEETHA RAMANI AND AMRITHA NAIR & K. KRITHIKA Serratia marcescens, optimization, antimicrobial activity. 383-392