10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) A novel actinomycetes strain designated KMA15, producing anticandidal substances was isolated from different locations in Coimbatore has been studied. Morphological and biochemical studies indicated that strain KMA15 belonged to the genus lessThan i greaterThan Streptomyces. lessThan /i greaterThan A total of 35 actinomycetes were subjected to agar disc method against lessThan i greaterThan Candida albicans. lessThan /i greaterThan It was observed that 12 isolates were active against lessThan i greaterThan Candida albicans. lessThan /i greaterThan The anticandidal substances were extracted by solvent extraction method with ethanol from isolate inoculated in yeast malt extract broth fermented for 7 days 28 lessThan sup greaterThan 0 lessThan /sup greaterThan C. Fungicidal activity was determined by observing clear zone, the actinomycetes strain KMA15 possessed great fungicidal activity for lessThan i greaterThan Candida albicans lessThan /i greaterThan by showing the 22mm of zone of inhibition.Minimum inhibitory concentration of the solvent extract was determined as 1.562 mg/ml against lessThan i greaterThan Candida albicans lessThan /i greaterThan . The antifungal substance active against the lessThan i greaterThan Candida albicans lessThan /i greaterThan were purified using column chromatography. The nature and further biological activity of the active principle are under investigation C. SWEETLINE, R. USHA AND M.PALANISWAMY Soil, Actinomycetes, Candida albicans, Anticandidal activity, Streptomyces 46-53