10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) The ethanolic leaf extracts of lessThan i greaterThan Solanum trilobatum lessThan /i greaterThan plant were used for antibacterial study by disc diffusion method. Different concentrations (10mg/ml, 20mg/ml, 30mg/ml) of the concentrated ethanolic leaf extract were tested for its antibacterial activity against bacterial strains such lessThan i greaterThan Bacillusubstilis, Staphylococcus aureus, Bacillus cereus lessThan /i greaterThan , lessThan i greaterThan E. Cali & Pseudomonas aeruginosa. lessThan /i greaterThan The bacterial cultures were grown in Muller hinton agar and Muller hinton broth. The zone of inhibition was comparatively more in the concentration of 30mg/ml. Phytochemical screening of the ethanolic leaf extract of the plant revealed the presence of tannins, saponins, flavonoids, carbohydrates and alkaloids. Thin Layer Chromatography (TLC) value of the leaf extract showed the highest Retention Factor (RF) value. The plant extracts showed better inhibitory activity against the tested organisms. PRIYA G AND CHELLARAM C Solanum trilobatum, bacterial strains, ethanol extract, TLC, phytochemicals, retention time 354-359