10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 3 2014 (July- September) In the present study the antibacterial activity of extracts and pure constituents of lessThan i greaterThan V. odorata lessThan /i greaterThan was evaluated against lessThan i greaterThan Mycobacterium tuberculosis lessThan /i greaterThan H37Rv and clinical isolate of MDR-TB ( lessThan i greaterThan M. avium, lessThan /i greaterThan ATCC 25291). Crude ethanol extract of the plant was fractionated with different solvents to yield respective extracts. The lessThan i greaterThan n lessThan /i greaterThan -hexane and Dichloromethane fractions showing good activity against both strains were purified by chromatographic techniques and prep-HPLC to yield several pure compounds characterized by LCMS. Seven isolated pure compounds were assayed against lessThan i greaterThan M. tuberculosis lessThan /i greaterThan H37Rv and MDR-TB isolate in different concentrations and their MIC's were recorded. Same concentrations were taken in case of MDR-TB isolate. Of the seven pure isolates two compound; salicin and phenyl alanine ethyl ester were reported in this plant as a new source. The structure of these compounds was established by comparison of spectral data with the one reported earlier. Results suggested that lessThan i greaterThan V. odorata lessThan /i greaterThan contained very active compounds against lessThan i greaterThan M. tuberculosis lessThan /i greaterThan H37Rv and lessThan i greaterThan M. avium lessThan /i greaterThan which can be used as lead for potential anti-TB drug formulations on further clinical trials. Ethno-medicinal use of lessThan i greaterThan V. odorata lessThan /i greaterThan by local Hakims for treating lung related problems was also supported. FAIZA HASSAN AND ISMAT NAEEM V. odorata, anti-TB activity, respiratory diseases. 61-69