10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) The experiment was design and conduct in control environmental condition for the purpose to investigate the impact of given metals Fe lessThan sup greaterThan 3+ lessThan /sup greaterThan and Cu lessThan sup greaterThan 2+ lessThan /sup greaterThan in the presence of nitrate and phosphate on the growth, protein level ( root, leaf, fruit ) production of photosynthetic pigments in macrophyte and its metal accumulation and removal capacity in the provided condition. lessThan i greaterThan T. natans lessThan /i greaterThan survive very well with Fe lessThan sup greaterThan 3+ lessThan /sup greaterThan in presence of nitrate and phosphate by increasing in biomass 1.17% more than the Cu lessThan sup greaterThan 2+ lessThan /sup greaterThan . In the protein level there is increment of 10.11%, 5%, 13.16% observed in leaf, root and fruit respectively. Photosynthetic pigments was observed 4.88% more in Fe lessThan sup greaterThan 3+ lessThan /sup greaterThan treated plant than that in Cu lessThan sup greaterThan 2+ lessThan /sup greaterThan treated plant and it was also noticed that bio accretion of Fe lessThan sup greaterThan 3+ lessThan /sup greaterThan was 8.8% more than of Cu lessThan sup greaterThan 2+ lessThan /sup greaterThan . Its resulted that given plant also played an important role in the elimination or purging of pollutant/metal ions (Fe lessThan sup greaterThan 3+ lessThan /sup greaterThan , Cu lessThan sup greaterThan 2+ lessThan /sup greaterThan ) in the maintenance of water quality, whereas in case of Fe lessThan sup greaterThan 3+ lessThan /sup greaterThan lessThan i greaterThan T. natans lessThan /i greaterThan also tolerated the biochemical and physiological stress and have a positive characteristic for the remedial process of polluted water. SAMEER CHANDRA , SATISH K. RAWAT , RANA P. SINGH AND SANJAY K. GARG T. natans, Biomass, Protein, Photosynthetic pigment, Metal ions 972-977