10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) Indolicidin is an antimicrobial cationic peptide naturally present in the bovine neutrophils. In this study, transformed lessThan i greaterThan Escherichia coli lessThan /i greaterThan cells were employed for greater and cost effective production of indolicidin. Four strains of lessThan i greaterThan E lessThan /i greaterThan . lessThan i greaterThan coli lessThan /i greaterThan [C41 (DE3), C43 (DE3), C41 (DE3) pLysS & C43 (DE3) pLysS] were transformed with recombinant plasmid pET21A lessThan sup greaterThan + lessThan /sup greaterThan carrying the indolicidin gene and were successfully expressed for indolicidin in the presence of the inducer isopropyl β-D-1-thiogalactopyranoside (IPTG). Further, the production of indolicidin was confirmed by detecting the 2 KDa indolicidin band on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). K. PANNEER SELVAM, B. ANNAMALAI, G. NAMBIRAJAN AND C.S. SHOBANA Indolicidin, E. coli, Transformation, Antimicrobial activity and pET21A 387-390