10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) The aim of present study was to evaluate the mutagenicity and genotoxicity induced due to medicinal plant lessThan i greaterThan Euphorbia royleana lessThan /i greaterThan crude latex in fresh water fish lessThan i greaterThan Channa punctatus lessThan /i greaterThan using micronucleus and single cell gel electrophoresis (SCGE) assays. Two sub-lethal concentration of crude latex were calculated using 24 hrs LC lessThan sub greaterThan 50 lessThan /sub greaterThan value and the fish specimens were exposed to these concentrations in a static system. Erythrocytes were sampled at the intervals of 24, 48, 72 and 96 hrs at the rate of five fish per interval for assessment of micronucleus induction and SCGE assay. Significant effects ( lessThan i greaterThan P lessThan /i greaterThan lessThan 0.05) for both concentration and time of exposure were observed in treated fish. Highest level of micronuclei induction in erythrocytes and DNA damage in the SCGE was at 96 hrs of exposure at all concentrations of crude latex. This study further confirmed that the micronucleus and SCGE assays are useful to determine potential genotoxicity of herbal formulation and might be appropriate as part of monitoring program. PRATIBHA SINGH, ANURAG DABAS, RASHMI SRIVASTAVA, N.S. NAGPURE AND AJAY SINGH Channa punctatus; Euphorbia royleana; Genotoxicity; Comet assay; DNA damage; Micronucleus test 217-228