10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) Fourteen actinomycetes were isolated from soil collected from five different coastal sites of Tamil Nadu. The actinomycete designated as "A" was selected as the most potent actinomycete. Among different solvents used, ethyl acetate was found to be more effective in extraction of bioactive compounds. Minimum Inhibitory Concentration of extract was found to be 35.6µg/ml for lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan . The actinomycete "A" strain had the ability to produce enzymes and the stability of extract was ideal at 8 lessThan sup greaterThan 0 lessThan /sup greaterThan C for 30 days. The stability of the extract was drastically reduced above 60 lessThan sup greaterThan 0 lessThan /sup greaterThan C and below pH 6. 16S rRNA sequence of the strain A showed 99% similarity with lessThan i greaterThan Streptomyces lessThan /i greaterThan sp. 2438. The accession number (JF751041) for the sequence of strain A was obtained from NCBI. Based on the phylogenetic evaluation, the actinomycete was designated as lessThan i greaterThan Streptomyces lessThan /i greaterThan lessThan i greaterThan dhinakaran lessThan /i greaterThan 2011, which was capable of producing extracellular bioactive metabolites, that inhibits the growth of lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan and other bacterial and fungal pathogens. S FEBINA BERNICE SHARON AND S KALIDASS Streptomyces dhinakaran 2011, Antimicrobial, Klebsiella pneumoniae, 16S rRNA sequence 158-164