10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) The aim of the present study was to evaluate the antimicrobial activity of lessThan i greaterThan Tinospora cordifolia lessThan /i greaterThan extracts against three standard ATCC strains of lessThan i greaterThan Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa lessThan /i greaterThan . The efficacy of extract was measured in terms of Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and zone of inhibition (mm). Antimicrobial activity of aqueous as well as ethanolic extracts were tested in vitro using standard microbroth dilution method with double dilution, against ATCC strains of lessThan i greaterThan E.coli, Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan for determination of MIC. MBC values were determined by subculture on standard solid media. The Kirby Bruer's Disk Diffusion Method was then used to observe the zones of inhibition to a range of concentration at MIC values with other broad spectrum antibiotics. Both aqueous and ethanolic extract of lessThan i greaterThan Tinospora cordifolia lessThan /i greaterThan showed activity against standard ATCC strains of lessThan i greaterThan E.coli, Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan in vitro at very high concentrations. Further studies are required to explore the antimicrobial efficacy of lessThan i greaterThan Tinospora cordifolia lessThan /i greaterThan against clinical isolates. DR. HANMANT AMANE,DR. SHILPA KAORE AND DR. NAVINCHANDRA KAORE Herbal extract, antibiotic, micro-organisms, resistance. 747-753