10.22376/ijpbs.2019.10.1.p1-12 Volume 1 Issue 3 2010 (July - September) A simple, precise and accurate high-performance liquid chromatographic method has been developed for simultaneous determination of β-sitosterol and Lupeol in whole plant powder of Vernonia cinerea Linn. Chromatographic separation of the two compounds was performed on a waters symmetry shield C18 column (150 x 4.6, 5 µm) as stationary phase with a mobile phase comprising of methanol : acetonitrile (30:70) v/v at a flow rate of 1.0 mL min-1 and UV detection at 210nm with a run time of 12.0 min. The proposed method was validated for linearity, accuracy, precision and limit of quantitation. The validated HPLC method can be used for a routine quality control analysis and simultaneous quantitation of β-sitosterol and Lupeol from Vernonia cinerea Linn.  Willy Shah,M. B. Kekare,Vikas Vaidya HPLC, β-sitosterol, Lupeol, Vernonia cinerea Linn. -