10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) The main objective of this study is antibacterial activity of Lemon ( lessThan i greaterThan Citrus medica lessThan /i greaterThan L.) and Bitter Orange ( lessThan i greaterThan Citrus aurantium lessThan /i greaterThan L.) Peel extract against clinical isolates from wound infection. As microorganism are becoming resistant to present day antibiotics, our study focuses on antimicrobial activity and future prophylactic potential of the lemon and orange peels. The antibacterial activity of Peel extract of lessThan i greaterThan Citrus medica lessThan /i greaterThan L. and lessThan i greaterThan Citrus aurantium lessThan /i greaterThan L. were evaluated on bacterial strains like lessThan i greaterThan E. coli, Ps. aeruginosa, Kl. pneumoniae, Pr. vulgaris, Serratia marcescens , Propionibacterium acne, Enterococcus spp., Streptococcus spp., S. aureus lessThan /i greaterThan and lessThan i greaterThan S. epidermidis. lessThan /i greaterThan All bacteria were isolated from wound infected patients and were multiple drug resistant to many antibiotics. Antibacterial activity of five different solvent extracts (Methanol, acetone, ethanol, petroleum ether and n-Hexane) were prepared by using Soxhlet extractor. In-vitro antibacterial activity was performed by agar well diffusion method. lessThan i greaterThan Citrus medica lessThan /i greaterThan peel extracts showed a very good antimicrobial activity when compared to lessThan i greaterThan Citrus aurantium lessThan /i greaterThan . Both plants peel extract showed considerable antibacterial activity against all tested bacteria. lessThan i greaterThan lessThan /i greaterThan The peel extract of lessThan i greaterThan Citrus lessThan /i greaterThan lessThan i greaterThan aurantium lessThan /i greaterThan and lessThan i greaterThan Citrus medica lessThan /i greaterThan can be considered to be as equally potent as the antibiotics used now-a-days TUMANE P.M , MESHRAM V. G AND WASNIK D. D Clinical isolates, antibacterial activity, wound infection, Lemon, Orange, Soxhlet extractor 382-387