10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 1 2014 (January - March) Biofilm formation of lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Staphylococcus epidermidis lessThan /i greaterThan were determined by the production of protease and lipase. Inhibition of lipolytic and proteolytic activity will lead to control of virulence factors produced by lessThan i greaterThan S. aureus lessThan /i greaterThan and lessThan i greaterThan S. epidermidis lessThan /i greaterThan . Antibiofilm activity of isolated compound Vancomycin against S lessThan i greaterThan . aureus lessThan /i greaterThan and lessThan i greaterThan S. epidermidis lessThan /i greaterThan showed significant results. The effect of Vancomycin on Hydrophobicity Index (HI), dispersion on biofilm formation by microscopic method and glycocalyx production shows complete eradication of the biofilm formation. Vancomycin used in this study showed biofilm dispersion and disruption of the biofilm of the architecture by reducing Exopolysaccharide (EPS) production and HI. The compound were studied for its wound healing property in rats and showed significant results compared to standard drug. The inhibitor used would have great clinical significance given to the difficulties encountered with treating lessThan i greaterThan S. aureus lessThan /i greaterThan and lessThan i greaterThan S. epidermidis lessThan /i greaterThan biofilm centered human infections RANJITH KUMAR M AND BRINDHA PRIYADARISINI V Biofilm formation, Vancomycin, wound healing, Skin infection 232-248