10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) The aim of the present investigation was to evaluate hepatoprotective activity of lessThan i greaterThan Nigella sativa lessThan /i greaterThan seed extract against diethyl phthalate (DEP) – induced toxicity in mice. Colony inbred Swiss strain adult female albino mice were orally administered with 310, 620 and 1240 mg/ kg body weight /day (low, mid, high dose respectively) for 30 days. Treatment caused, as compared with the control, significant (p lessThan 0.05) decrease in body weight and significant (p lessThan 0.05) increase in absolute and relative weights of liver. Also, DEP treatment caused significant (p lessThan 0.05), dose – dependent increase in lipid peroxidation in liver and liver marker enzyme activities measured in serum such as ACP, ALP, AST and ALT than that of vehicle control. Histopathological studies revealed, intracellular vacuolation, necrosis and loss of hepatic architecture in DEP - treated animals. lessThan i greaterThan Nigella sativa lessThan /i greaterThan seed extract (150 and 300 mg/kg body weight/day) treatment along with HD of DEP , caused significant (p lessThan 0.05) restoration in body weight, organ weight, lipid peroxidation in liver as well as liver marker enzymes in serum as compared to DEP alone treated mice. In addition histopathological changes were completely ameliorated in high dose extract treated mice. It is concluded from the present study that supplementation of lessThan i greaterThan Nigella sativa lessThan /i greaterThan extract can be beneficial in positively modulating DEP – induced alterations in liver. HEENA PRAJAPATI AND RAMTEJ J. VERMA Diethyl phthalate, enzymes, hepatotoxicity, histopathology, liver, Nigella sativa 1366-1376