10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) To optimize the production of gamma linolenic acid by lessThan i greaterThan Mortierella rammaniana lessThan /i greaterThan Methodology and Result: Effect of carbon source, nitrogen source, initial pH and oil supplementation was studied. The production of gamma linolenic acid reached 920 m gl lessThan sup greaterThan -1 lessThan /sup greaterThan when the production media were composed of 10% glucose, 1% peptone and 0.1% yeast extract pH 6.5 and incubated at 30 lessThan sup greaterThan áµ’ lessThan /sup greaterThan C for 144 hrs. Carbon and nitrogen sources showed major effect on the increase in gamma linolenic acid production. Repression in Δ 6-desaturase activity was observed in the presence of supplemented oil. This study highlights conditions for increasing gamma linolenic acid production by lessThan i greaterThan Mortierella rammaniana lessThan /i greaterThan and an insight into rapidly gaining high production of polyunsaturated fatty acids. ANANIA ARJUNA Lipid; gamma; Linolenic acid; Mortierella ramanniana; Fatty acid; culture 1038-1048