10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) A novel fibrinolytic enzyme producing bacteria was isolated and identified by morphological and biochemical characteristics and confirmed by 16S r DNA as lessThan i greaterThan Lysinibacillus xylanilyticus. lessThan /i greaterThan The optimum conditions for enzyme production was determined by response surface methodology instead of one factor at a time method. Placket burman method was used for the initial screening process. In our study the newly isolated strain lessThan i greaterThan Lysinbacillus xylanyliticus lessThan /i greaterThan was found to produce fibrinolytic enzyme (2468.713U/ml) using the statistically optimized media which contain glucose, soy peptone, magnesium sulphate and ammonium nitrate as components. RESHMA C V AND FATHIMATHU ZUHARA K fibrinolytic enzyme, Lysinibacillus xylanilyticus, response surface methodology 1030-1037