10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) The aim of the present study was to evaluate antimicrobial activity of lessThan i greaterThan Ganoderma applanatum, Tricholoma giganteum lessThan /i greaterThan and lessThan i greaterThan Bolbitius vitellinus lessThan /i greaterThan . The methanolic extract of basidiocarps and mycelial mat of all the above mentioned mushrooms was analyzed. The methanolic extracts were tested against three Gram positive, six Gram negative bacteria and two fungal cultures. Out of three mushrooms lessThan i greaterThan Ganoderma applanatum lessThan /i greaterThan was found to be more active against bacterial cultures, followed by lessThan i greaterThan Tricholoma giganteum lessThan /i greaterThan and lessThan i greaterThan Bolbitius vitellinus lessThan /i greaterThan . lessThan i greaterThan G.applanatum lessThan /i greaterThan and lessThan i greaterThan T.giganteum lessThan /i greaterThan was found to be more sensitive to Gram Positive bacteria than Gram negative bacteria, however lessThan i greaterThan B.vitellinus lessThan /i greaterThan was found to very active against Gram negative bacteria especially lessThan i greaterThan E.coli. B.vitellinus lessThan /i greaterThan showed highest antifungal activity against lessThan i greaterThan Candida albicans, lessThan /i greaterThan other two mushrooms. Hence these mushrooms can be exploited for their useful metabolites in Food and Pharmaceutical industries. PUSHPA.H, ANAND.M, KASIMAIAH.P AND PENUGONDA.J.V.S.PRADEEP Ganoderma applanatum, Tricholoma giganteum, Bolbitius vitellinus, Antimicrobial activity. 964-971