10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) In the present study we collected seven blood samples from patients infected with lessThan i greaterThan M. pneumonia. lessThan /i greaterThan Genomic DNA was isolated from these blood samples and specific primers were designed for lessThan i greaterThan p lessThan /i greaterThan 1 gene coding for cytadhesin protein P1 of the lessThan i greaterThan M. pneumonia. lessThan /i greaterThan The amplified product was cloned into pTZ57R/T cloning vector and transformed into lessThan i greaterThan E. coli lessThan /i greaterThan strain DH5α. Plasmid was isolated from the transformed cells, digested and checked for gene product release. Also, colony PCR was used to confirm the cloning and transformation. The released gene product was eluted and sequenced. The obtained sequence was 99% matching with a part of lessThan i greaterThan p lessThan /i greaterThan 1 gene available in the public nucleotide date base. In conclusion, this report describes a genomics-based PCR that provided rapid detection of lessThan i greaterThan M. pneumoniae lessThan /i greaterThan . This PCR assay was developed for testing blood specimens suspected to harbor lessThan i greaterThan M. pneumoniae lessThan /i greaterThan and is highly sensitive. ABOO THAR ALI HAMMADI AND SONIA SHARMA M. pneumonia, p1 gene, PCR, Cloning, Sequencing 770-774