10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) Snake venom is a rich source of metalloproteinase which catalytic mechanism involves a metal after snake bite is an important factor for created disease such as necrosis, heart disease, liver disease. However, this is a very much important protein to design drug for treatment of various diseases. In this study first we compared the amount of essential amino acids Histidine (H), Isoleucine (I), Leucine (L), Lysine (K), Methioninne (M), Phenylalanine (F), Threonine (T), Tryptophan (W) and Valine (V) in metalloproteinase four rattle snake where it was more (47.6%) lessThan i greaterThan lessThan /i greaterThan in lessThan i greaterThan Crotalus adamanteus lessThan /i greaterThan and less (43.3%) in lessThan i greaterThan Sistrurus catenatus edwardsi lessThan /i greaterThan . The multiple sequence alignment showed the highest similarity between lessThan i greaterThan C. v. viridis lessThan /i greaterThan and lessThan i greaterThan C. d. durissus lessThan /i greaterThan (97%) and the lowest between lessThan i greaterThan C. adamanteus lessThan /i greaterThan and lessThan i greaterThan S. c. edwardsi lessThan /i greaterThan (69%). Secondly we compared the percentage of essential amino acids in metalloproteinase of rattle snake lessThan i greaterThan C. adamanteus lessThan /i greaterThan with the Asian snake lessThan i greaterThan Naja atra lessThan /i greaterThan (40.95%) and their similarities was 55%. So rattle snake lessThan i greaterThan C. adamanteus lessThan /i greaterThan contains more essential amino acids in metalloproteinase. This result indicates that metalloproteinase could be useful to develop target drug after isolation from venomous rattle snake species lessThan i greaterThan C. adamanteus lessThan /i greaterThan . MD. MAHMUDUL ISLAM, MD. ENAMUL HAQUE, AMIT KUMAR DUTTA, DR. MD. ASADUL ISLAM,DR. MD. KHALEKUZZAMAN AND DR. BISWANATH SIKDAR Metalloproteinase, ProtParam, Sequence, Alignment, Venomous 741-748