10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) The aim of this study was to evaluate the cytotoxic and apoptotic effects of chloroform extract (ASCH) from the seeds of lessThan i greaterThan Annona squamosa. lessThan /i greaterThan Materials and Methods: MTT assay was employed to determine the cytotoxicity of the extract on A-549 and EAC cells and IC lessThan sub greaterThan 50 lessThan /sub greaterThan values were calculated. The apoptotic effects induced by the ASCH was evaluated by Acridine Orange - Ethidium Bromide dual staining and Hoechst staining by Fluorescence microscopy. Results: The ASCH extract 400 µg/ml exhibited maximum cytotoxic effects of 54±1.2 and 56± 0.9 at 48 hours on human Lung adenocarcinoma cells (A-549) and murine tumor (EAC) cells respectively. IC lessThan sub greaterThan 50 lessThan /sub greaterThan values were 319 µg/ml and 312 µg/ml for A-549 cells and EAC cells respectively. Acridine Orange Ethidium Bromide and Hoechst staining exhibited apoptotic features such as chromatin condensation and nuclear fragmentation after treatment with the extract. Conclusions: These results suggest that the chloroform extract from lessThan i greaterThan Annona lessThan /i greaterThan lessThan i greaterThan squamosa lessThan /i greaterThan seeds have cytotoxic and apoptotic effect. BIBA VEERABHADRAN SOBHANA AND REMANI PRATHAPAN Annona squamosa, murine cell, Apoptosis, Cytotoxicity 682-687