International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 4
2013 (October - December)
CYTOTOXIC AND APOPTOTIC EFFECT OF ANNONA SQUAMOSASEED EXTRACT ON HUMAN AND MURINE TUMOR CELLS.
The aim of this study was to evaluate the cytotoxic and apoptotic effects of chloroform extract (ASCH) from the seeds of lessThan i greaterThan Annona squamosa. lessThan /i greaterThan Materials and Methods: MTT assay was employed to determine the cytotoxicity of the extract on A-549 and EAC cells and IC lessThan sub greaterThan 50 lessThan /sub greaterThan values were calculated. The apoptotic effects induced by the ASCH was evaluated by Acridine Orange - Ethidium Bromide dual staining and Hoechst staining by Fluorescence microscopy. Results: The ASCH extract 400 µg/ml exhibited maximum cytotoxic effects of 54±1.2 and 56± 0.9 at 48 hours on human Lung adenocarcinoma cells (A-549) and murine tumor (EAC) cells respectively. IC lessThan sub greaterThan 50 lessThan /sub greaterThan values were 319 µg/ml and 312 µg/ml for A-549 cells and EAC cells respectively. Acridine Orange Ethidium Bromide and Hoechst staining exhibited apoptotic features such as chromatin condensation and nuclear fragmentation after treatment with the extract. Conclusions: These results suggest that the chloroform extract from lessThan i greaterThan Annona lessThan /i greaterThan lessThan i greaterThan squamosa lessThan /i greaterThan seeds have cytotoxic and apoptotic effect.
BIBA VEERABHADRAN SOBHANA AND REMANI PRATHAPAN
Annona squamosa, murine cell, Apoptosis, Cytotoxicity
682-687